human sat antibody  (R&D Systems)

Journal: Communications Biology

Article Title: Nasal symbiont Staphylococcus epidermidis restricts influenza A virus replication via the creation of a polyamine-deficient cellular environment

doi: 10.1038/s42003-024-06706-4

Figure Lengend Snippet: A Schematic mechanism of the depletion of polyamines by DENSpm, B SAT1 protein expression, C Alterations of polyamine metabolism-related metabolites, D , E viral PA mRNA expression by treating DENSpm or synthetic polyamines, and F viral NP protein expression. DENSpm-N1 N11-diethylnorspermine, SAT1 spermidine/spermine N1, acetyltransferase, PA polymerase, NA neuraminidase.

Article Snippet: Human ODC1 antibody (molecular weight 61 kDa, cat# MAB2695-SP, primary antibody 1:500), anti-β-actin antibody (molecular weight 43 kDa, primary antibody 1:500), and human SAT1 antibody (molecular weight 20 kDa, cat #AF-1786) were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Expressing

Journal: Cancers

Article Title: Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways

doi: 10.3390/cancers12010138

Figure Lengend Snippet: DSF/Cu inhibits nasopharyngeal carcinoma cells via the ferroptosis pathway. ( A ) Western blot analysis for the expression of p53-related proteins in 5-8F treated with DSF/Cu (1 μM/1 μM); the p53 inhibitor Pifithrin-α (20 μM) was pretreated for 12 h. Data are shown as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, n = 3. ( B , C ) RT-qPCR analysis of Ptgs2 and SAT1 mRNA levels in 5-8F. Data are shown as means ± SD. * p < 0.05, *** p < 0.001 vs. control group, n = 3. ( D ) The expression of SAT1 was detected by Western blotting in 5-8F, after cultured with DSF/Cu (1 μM/1 μM). Data are shown as means ± SD. *** p < 0.001, n = 3. ( E ) RT-qPCR analysis of ALOX15 mRNA levels in 5-8F. Data are shown as means ± SD. ** p < 0.01, *** p < 0.001 vs. control group, n = 3. ( F ) Lipid ROS production in 5-8F treated with DSF/Cu (1 μM/1 μM) for 6 h was assessed by flow cytometry using C11-BODIPY. NAC was pretreated for 12 h. Data are shown as means ± SD. *** p < 0.001, n = 3. ( G ) The percentage of viable 5-8F cells after being treated with DSF/Cu (1 μM/1 μM) for 24 h. Inhibitors were pretreated for 12 h. DMSO solvent was used as a control. Data are shown as means ± SD. *** p < 0.001 vs. DSF/Cu group (the second group), n = 3.

Article Snippet: Total cell proteins were separated by 10% SDS-PAGE, transferred to polyvinylidene difluoride membranes, and probed with antibodies directed against human PARP, Caspase3, Cleaved-Caspase3, p-JNK, p38, p-p38 and BAX (1:1000, Cell Signaling Technologies, CST, Boston, MA, USA), JNK (1:500, Wanleibio, Shenyang, China), p53 and p21 (1:1000, proteintech, Chicago, IL, USA), ALDH1A1 and ALDH2 (1:100, Boster, Wuhan, China), SAT1 (1:500, Elabscience, Wuhan, China), α-SMA (1:100, Boster, Wuhan, China), and FAP-α (1:400, R&D Systems, Minneapolis, MN, USA).

Techniques: Western Blot, Expressing, Quantitative RT-PCR, Cell Culture, Flow Cytometry